1. Keep the scaffold in the plastic and sterilize using one of the two options:
a) Use dry heat with settings: 130°C for 2 hours.
Do not use temperature higher than 150°C.
b) Use UV radiation.
2. Remove the scaffold from the plastic in sterile environment.
3. Use a punch tool to cut discs with desired diameter.
4. Secure the discs into the bottom of the well.