GUIDELINES

1. Keep the scaffold in the plastic and sterilize using one of the two options:
   

       a) Use dry heat with settings: 130°C for 2 hours. 
           Do not use temperature higher than 150°C.

       b) Use UV radiation.

2. Remove the scaffold from the plastic in sterile environment.

3. Use a punch tool to cut discs with desired diameter. 

4. Secure the discs into the bottom of the well. 

5. Culture!